This study systematically expanded the EcN-based toolbox, enabling EcN as a powerful platform for more applications and developing EcN native integrase-mediated genetic engineering capabilities and establishing an in vitro cell-free protein synthesis system. Escherichia coli Nissle 1917 (EcN) is a probiotic microbe that has the potential to be developed as a promising chassis for synthetic Singh AK, Pandey SK, Naresh KG. Pyrroloquinoline quinone-secreting probiotic Escherichia coli Nissle 1917 ameliorates ethanol-induced oxidative damage and hyperlipidemia in rats. Alcoholism, Clinical and Experimental Research. 2014; 38:2127-2137. DOI: 10.1111/acer.12456 However, the extremely short half-life of GLP-1 due to degradation by the ubiquitous proteolytic enzyme limits its clinical application. In this study, we engineered the recombinant integrant probiotic strain Escherichia coli Nissle 1917 (EcN) to create a strain EcN-GLP-1 that effectively delivers the heterologous GLP-1 molecule. Subsequently The probiotic Escherichia coli strain Nissle 1917 (DSM 6601, Mutaflor), generally considered beneficial and safe, has been used for a century to treat various intestinal diseases. However, Nissle 1917 hosts in its genome the pks pathogenicity island that codes for the biosynthesis of the genotoxin colibactin. Colibactin is a potent DNA We hypothesized that the engineered probiotic harboring hlpA and azurin genes possessed an antitumor effect. In this study, an anti-CRC therapeutic bacteria Ep-AH was developed using E. coli Nissle 1917. We aimed to examine whether Ep-AH could protect colon against AOM/DSS, suppress tumorigenesis and modulate gut microbiota in the AOM/DSS mouse Here we compared the efficiency of tumor-specific colonization of pathogenic Salmonella typhimurium strains 14028 and SL1344 to the enteroinvasive Escherichia coli 4608-58 strain and to the attenuated Salmonella flexneri 2a SC602 strain, as well as to the uropathogenic E. coli CFT073, the non-pathogenic E. coli Top10, and the probiotic E. coli Aims: This study was prompted to investigate the intestinal localization and colonization of orally administered Escherichia coli Nissle 1917 (EcN) in piglets. Methods and Results: EcN was fed to ten EcN-negative piglets (3 months) over seven consecutive days. Faecal samples were collected repeatedly and tested for EcN-DNA by a combined culture As a result, traditional probiotic bacterial strains such as E. coli Nissle 1917 and lactic acid bacteria are the typical choice for engineering and deployment into the gut. E. pxB1Pp.

e coli nissle 1917 probiotic